Journal: PLoS ONE
Article Title: Growth and Differentiation Factor 3 Induces Expression of Genes Related to Differentiation in a Model of Cancer Stem Cells and Protects Them from Retinoic Acid-Induced Apoptosis
doi: 10.1371/journal.pone.0070612
Figure Lengend Snippet: A–G. Effect of stimulation with different GDF3 concentrations on transcription of several genes regulated on the microarrays A & B. n = 5. H. Effect of GDF3 knockdown on transcription of several genes regulated on the microarray E. n = 3. The expression was measured by qPCR, the results are presented as GAPDH ratio and normalized to unstimulated cells (A–G) or cells transduced with scrambled-vector (H). P-values smaller than or equal to 0.05 were considered significant. (*) indicates p≤0.05 and (**) p≤0.01. Data is represented as means +/− standard deviation.
Article Snippet: For the transcriptome analysis human genome CGH Microarray 44K (Agilent Technologies, USA) was utilized according to manufactures protocol.
Techniques: Microarray, Expressing, Transduction, Plasmid Preparation, Standard Deviation